Bio-functionalized stimulus-responsive dissolvable PEG-hydrogels

摘要

The present invention is directed to a bio-functionalized stimulus-responsive dissolvable PEG-hydrogel. This inventive stimulus-responsive dissolvable PEG-hydrogel comprises a matrix of PEG-polymers, which are modified to contain at least one multifunctional fusion protein, the multifunctional fusion protein preferably comprising as components a substrate binding peptide (SBP), preferably a repetitive RGD-binding peptide and/or a ZZ-binding domain, preferably a tag for purification, and at least one N- and/or C-terminal linker. The present invention is furthermore directed to the use of such inventive stimulus-responsive dissolvable PEG-hydrogels in the treatment of lesions, in surgical dressings, for wound treating, for soft and hard tissue regeneration, for the treatment of wounds in the oral cavity, in the field of ophthalmology, in the field of periodontal defects, etc. The invention also describes a method of treatment for such diseases. Additionally, the present invention provides a kit comprising the inventive stimulus-responsive dissolvable PEG-hydrogel and optionally further components.

主权项

1. A stimulus-responsive dissolvable poly(ethylene glycol) hydrogel (PEG-hydrogel) comprising a matrix of PEG-polymers, which are modified to contain at least one multifunctional fusion protein, the multifunctional fusion protein comprising as components a single chain variable fragment (scFv fragment), a repetitive arginine-glycine-aspartic acid binding peptide (RGD-binding peptide), and at least one N- and/or C-terminal linker, wherein the at least one multifunctional fusion protein is covalently bound to the PEG polymer, and wherein the scFv fragment is the scFv fragment according to SEQ ID NO: 69 or the amino acid sequence showing at least 97.5% identity to said sequence.