Hybrid and tandem expression of neisserial proteins

摘要

Two or more Neisserial proteins are joined such that they are translated as a single polypeptide chain. Hybrid proteins are represented by the formula NH2-A-[-X-L-]n-B—COOH where X is an amino acid sequence, L is an optional linker amino acid sequence, A is an optional N-terminal amino acid sequence, B is an optional C-terminal amino acid sequence, and n is an integer greater than 1. Proteins where each of the n -X- moieties shares sequence identity to each other -X- moiety, the protein is a ‘tandem protein’.

主权项

1. The hybrid protein comprising formula:
NH2-A-[-X-L-]n-B—COOHwherein L is an optional linker amino acid sequence, A is an optional N-terminal amino acid sequence, B is an optional C-terminal amino acid sequence, and n is an integer greater than 1 and an X1 portion of the NH2-A-[-X-L-]n-B—COOH formula comprises an amino acid sequence having at least 90% identity to SEQ ID NO: 2206 and an X2 portion of the NH2-A-[-X-L-]n-B—COOH formula comprises an amino acid sequence having at least 90% identity to SEQ ID NO: 2207.