| 摘要 |
The present invention is related to the use of Botrytis cinerea strains, its spores, hyphae mycelium, sclerotia, intra and/or extracellular organic molecules, such as proteins, nucleic acids, polysaccharides, lipids and secondary metabolites for the biosynthesis of gold nanoparticles (AuNps). In general terms, the present invention is focused to use B. cinerea strains and/or molecules generated by this organism for the biological synthesis of AuNps, being then the field of application, the synthesis of nanomaterials, specifically AuNps using the phytopathogenic fungus B. cinerea and/or its intra or extracellular proteins purified individually or in combination thereof or any of other intra and/or extracellular molecule produced by this organism as a biological system of synthesis. The metallic nanoparticles are used in various applications including: semiconductors, photoluminescence, biomedicine, imaging for the medical diagnostic, catalysts (dispersed and supported) and in therapies against some types of neoplasia (cancer), among others. |
| 主权项 |
1. Process for the biological synthesis of gold nanoparticles (AuNps), wherein it comprises:
a) mycelium culturing of Botrytis cinerea fungal in a nutritive medium containing between 0.1-1% malt extract and 0.1-1% yeast extract, at 20° C. in darkness for at least 10 days; and b) Generating gold nanoparticles (AuNps), taking the supernatant obtained from the step a) and incubating it with HAuCl4•3H2O for a period of time ranging between 0.5 to 12 hours, at a temperature ranging between 25-27° C., and retrieving the nanoparticles by low-speed centrifugation or by spontaneous sedimentation after a rest of at least 1 hour. |
