| 主权项 |
1. A system for correcting the color of transmission microscope images for different illuminants, comprising:
a sample; a microscope having:
a configurable numerical aperture having a plurality of pre-set positions,an optical train having a distal end and a proximal end and configured to convey illumination,an illumination source configured to direct illumination into the distal end of the optical train, anda selectable end piece positioned at the proximal end of the optical train, wherein the end piece is configured to direct the illumination to one of an image recording device or a removable light spectrum sensing device; a microscope slide having:
an integral transmission filter array of known color spectra for each position of the numerical aperture and selectively disposed between the illumination source and the optical train; an image recording device, configured to record a plurality of images of the slide and the integral color filter array, wherein a raw image is comprised of a pixel array, each pixel having a color value; an image processor, configured by code executing therein, to:
select a destination illumination;calculate the tristimulus values of the color filter array;obtain the raw image from the image recording device of the slide sample and color filter array;extract the RGB pixel values of the color filter array from the raw image;create a mapping matrix of the RGB pixel values to the tristimulus values;generate tristimulus values of a composite image by applying the mapping matrix to the RGB pixel values of the raw image; whereby the color of the microscope images is corrected without user intervention. |
