| 摘要 |
<p>#CMT# #/CMT# Measuring real-time secretion of at least one compound by at least one individual cell, comprises: culturing at least one cell in a culture chamber comprising at least one wall with at least one sensitive area, where the sensitive area comprises at least one ligand attached to a solid support, the ligand is capable of binding specifically to the compound and an element for real-time measurement of a signal produced by the binding of the compound to the ligand; identifying at least one spot emitting a signal in sensitive area; and measuring real-time the signal produced by identified spot. #CMT# : #/CMT# Measuring real-time secretion of at least one compound by at least one individual cell, comprises: culturing at least one cell in a culture chamber comprising at least one wall with at least one sensitive area, where the sensitive area comprises at least one ligand attached to a solid support, the ligand is capable of binding specifically to the compound and an element for real-time measurement of a signal produced by the binding of the compound to the ligand; identifying in the sensitive area, at least one spot emitting a signal produced by the binding of at least one compound respectively to at least one ligand; and measuring real-time the signal produced by the spot identified, where the signal represents the amount of compound secreted by an individual cell. #CMT#USE : #/CMT# The method is useful for measuring real-time secretion of a compound by at least one individual cell (claimed), where the cell is bacterial, fungal, algal, protozoan or metazoan, and originates from biological sample, food sample, water sample, soil sample, sludge sample or air sample, the biological sample is blood, cerebrospinal fluid, oral secretion, sperm, a vaginal secretion, urine, feces, synovial fluid, biopsy, specimen originating from lavage of organ or of anatomical cavity, sample from drainage of biological fluid, specimen of cutaneous or conjunctival serous fluid, the cell is immune cell, nerve cell, endocrine cell, stem cell, epithelial cell, cell infected with an infectious agent or cancer cell, and the compound is a protein, polypeptide, peptide, lipid, glycoprotein, glycolipid, lipoprotein, inorganic ion, small organic molecule, vesicle, microbial particle or viral particle, intercellular signaling compounds, extracellular matrix protein, immunoglobulin, and infectious agent or subunit of an infectious agent (all claimed). #CMT#ADVANTAGE : #/CMT# The method provides signal directly mediated by molecules present in the culture chamber. #CMT#BIOLOGY : #/CMT# Preferred Components: The ligand is a peptide, a polypeptide, a protein, a glycoprotein, an oligosaccharide, a polysaccharide, a nucleic acid, a lipid, a polymer or an organic compound comprising 1-100C. The ligand is an antibody, an antibody fragment, single-chain variable fragments, an antigen, a hapten, an aptamer, a lectin or a chelating agent. The ligand density in the sensitive area is 10 8>-10 1> 2>ligands/mm 2>. #CMT#INSTRUMENTATION AND TESTING : #/CMT# Preferred Method: The signal is measured using a near-field optical technique, preferably surface plasmon resonance. Preferred Components: The wall of the culture chamber is a basal wall in which the cells can sediment, and the amount of cells in the culture chamber is 1-10 6>cells/mm 2>. #CMT#POLYMERS : #/CMT# Preferred Components: The solid support is a material comprising glass, silicon, an organic polymer, a metal material or a carbon-based conductor.</p> |
