| 摘要 |
<p>Disclosed is a method for adjusting the amplification efficiency of a target polynucleotide in the amplification of the target polynucleotide by PCR using primers (i) to (iii) below, the method comprising adjusting the amplification efficiency of the target polynucleotide by changing the quantity ratio of the primers (i) to (iii) below:
(i) a first primer which is able to be base-paired with the target polynucleotide;
(ii) a second primer which is able to be base-paired with the target polynucleotide in competition with the first primer and from which an extension reaction by PCR occurs to a lesser extent as compared to the first primer; and
(iii) a third primer designed to allow for the amplification of the target polynucleotide in pairs with the first primer.</p> |
