Substrate mimicking intercellular lipids in stratum corneum and method of evaluating skin roughening using the same

摘要

A substrate mimicking intercellular lipids in stratum corneum consisting of a substrate and a lipid membrane formed on the substrate, wherein the lipid membrane is formed from ceramide, palmitic acid and cholesterol, and the ceramide, palmitic acid and cholesterol are present at a mass ratio of 20-70%:10-60%:20-40% (ceramide:palmitic acid:cholesterol).

主权项

1. An in vitro method for evaluating a potency of a
surfactant for inducing skin roughening, comprising: adding a surfactant to an engineered substrate model mimicking intercellular lipids in stratum corneum, the engineered substrate model consisting of a non-biological substrate, and a lipid membrane formed on the substrate so as to have a lamellar structure, the lipid membrane comprising ceramides, palmitic acid, and cholesterol; visualizing for a myelin figure on the engineered substrate by microscope, and; judging: the surfactant as causing skin roughening in vivo when the myelin figure is formed on the engineered substrate, and wherein the surfactant is more potent for inducing skin roughening when the shortest distance between the tip of the myelin figure and an edge of the engineered substrate is longer.