Compound arrays for sample profiling

摘要

The invention provides arrays of compound for use in profiling samples. The arrays include compounds bind to components of the samples at relatively low affinities. The avidity of compounds binding to components of the samples can be increased by forming arrays such that multivalent components of the samples (e.g., antibodies or cells) can bind to more than one molecule of a compound at the same time. When a sample is applied to an array under such conditions, the compounds of the array bind to component(s) of the sample with significantly different avidities generating a profile characteristic of the sample.

主权项

1. A method of determining a status of a subject, comprising:
(a) contacting a sample from the subject comprising at least 100 different antibodies, wherein not all of the at least 100 different antibodies are autoantibodies, with an array of immobilized different peptides of between 10-30 amino acids in length, wherein each different peptide has a unique amino acid sequence, each of the immobilized different peptides occupying different features of the array, wherein at least 50 of the immobilized different peptides have dissociation constants of between 1 mM and 1 μM to at least one antibody in the sample, and wherein the array comprises at least 100,000 immobilized different peptides; (b) detecting binding of the immobilized different peptides in the array to antibodies of the sample to obtain a binding profile of the sample, wherein the binding profile is the aggregate effect of the sample binding to the immobilized different peptides occupying different features of the array; and (c) analyzing the binding profile by comparing the binding profile detected to a reference binding profile to determine the status of the subject.