主权项 |
1. A method of selecting a population of plants or seeds with a high energy use efficiency comprising the steps of:
a. providing a population consisting of a plurality of individual plants which are genetically uniform; b. isolating a tissue sample or explant from individual plants of said population in a manner which allows further cultivation of said sampled individual plants; c. optionally, culturing said tissue samples or explants under conditions which activate the metabolism in said plants; d. determining the cellular respiration rate of said individual plants by analyzing said samples of said plants; e. selecting a number of plants wherein said sample exhibits a cellular expiration which is lower, preferably is significantly lower, than the average cellular respiration of samples from said population; f. growing the selected plants and propagating from each of the selected plants a line of cloned progeny plants; g. determining the energy use efficiency for each line of cloned progeny plants; h. selecting a line of clone plants wherein said energy use efficiency is higher, than the average of the energy use efficiency of all lines of cloned progeny plants, preferably select the line of clone progeny plants with the highest energy use efficiency; i. growing a population of individual plants from said selected line of clone progeny plants; and j. reiterating at least once steps b to i on said subsequent population. |