发明名称 |
SYSTEM AND METHOD FOR AUTOMATED NUCLEIC ACID AMPLIFICATION |
摘要 |
An embodiment of a device for automatically executing a process of generating an emulsion containing nucleic acids, amplifying the nucleic acids in the emulsion, breaking the emulsion, and separating and purifying said amplified nucleic acids, is described that comprises an emulsion generation unit for sealing beads to which nucleic acids are bound in a water-in-oil type emulsion; a nucleic acid amplification unit provided with a reaction vessel for amplifying said nucleic acids and a heating and cooling part for heating and cooling the reaction vessel; an emulsion breaking unit for breaking the emulsion after nucleic acid amplification; and a nucleic acid purification unit for recovering said amplified nucleic acids from said emulsion breaking unit. |
申请公布号 |
US2015056663(A1) |
申请公布日期 |
2015.02.26 |
申请号 |
US201414467321 |
申请日期 |
2014.08.25 |
申请人 |
454 Life Sciences Corporation |
发明人 |
TAJIMA HIDEJI;UEDA TETSUYA;KOBAYASHI SHUICHI;KANE NATHAN ROBERT |
分类号 |
B01L7/00;B01L3/00 |
主分类号 |
B01L7/00 |
代理机构 |
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代理人 |
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主权项 |
1. A device for automatically executing a process of generating an emulsion containing nucleic acids, amplifying the nucleic acids in the emulsion, breaking the emulsion, and separating and purifying said amplified nucleic acids, said device comprising:
an emulsion generation unit for sealing beads to which nucleic acids are bound in a water-in-oil type emulsion; a nucleic acid amplification unit provided with a reaction vessel for amplifying said nucleic acids and a heating and cooling part for heating and cooling the reaction vessel; an emulsion breaking unit for breaking the emulsion after nucleic acid amplification; and a nucleic acid purification unit for recovering said amplified nucleic acids from said emulsion breaking unit. |
地址 |
Branford CT US |