Purification and use of a factor for supporting wound healing

摘要

A process for manufacturing of a composition containing a purified factor for supporting wound healing selected from the group consisting of Hepatocyte Growth Factor (HGF) platelet derived growth factor (PDGF), Epidermal growth factor (EGF), transforming growth factor alfa (TGF-α), Transforming growth factor beta (TGF-β), insulin like growth factor (IGF-I) and Fibroblast growth factor (FGF) from sources, such as blood, containing the factor for supporting wound healing, wherein the manufacturing process comprises purification steps which are performed in the presence of antithrombin III (AT-III).

主权项

1. A method of purifying a factor for supporting wound healing selected from the group consisting of hepatocyte growth factor (HGF), platelet derived growth factor (PDGF), epidermal growth factor (EGF), transforming growth factor alpha (TGF-α), transforming growth factor beta (TGF-β), and insulin like growth factor (IGF-I) from a source containing said factor, wherein all purification steps of the method are performed in the presence of antithrombin III (AT-III) and the method comprises:
(i) thawing a frozen source containing the factor for supporting wound healing, removing a precipitate of the thawed source, and further processing a supernatant of the thawed source containing the factor for supporting wound healing; (ii) contacting a solution containing the supernatant and AT-III with a heparin-affinity chromatography material; (iii) separating the solution from the heparin-affinity chromatography material and contacting the heparin-affinity chromatography material with a desorption buffer having an ionic strength sufficient to desorb the factor for supporting wound healing from the heparin-affinity chromatography material; and (iv) collecting the desorption buffer containing the factor for supporting wound healing, AT-III, and histidine-rich glycoprotein (HRGP).