| 发明名称 | Method for purifying the rabies virus | ||
| 摘要 | The subject of the invention is a method for purifying the rabies virus, comprising a single ion-exchange chromatography step, said step being cation exchange chromatography according to which: a) the supernatant of a culture of cells infected with this virus is brought into contact with a cation exchange chromatography support comprising a polymethacrylate matrix onto which sulfoisobutyl groups have been grafted such that the rabies virus binds to this support, and;b) the virus is eluted from its support. | ||
| 申请公布号 | US8961997(B2) | 申请公布日期 | 2015.02.24 |
| 申请号 | US201012756590 | 申请日期 | 2010.04.08 |
| 申请人 | Sanofi Pasteur SA | 发明人 | Fabre Virginie;Rocca Céline;Riffard Pierre;Calvosa Eric |
| 分类号 | B01D15/08;B01D15/00;C02F1/42;A61K39/205;C12N7/00;A61K39/12 | 主分类号 | B01D15/08 |
| 代理机构 | McDonnell Boehnen Hulbert & Berghoff LLP | 代理人 | McDonnell Boehnen Hulbert & Berghoff LLP |
| 主权项 | 1. A method of purifying rabies virus, the method comprising a single ion exchange chromatography purification wherein said ion exchange chromatography purification is a cation exchange chromatography purification comprising a) applying a supernatant of a culture of cells infected with the rabies virus to a cation exchange chromatography support under conditions in which the rabies virus binds to the support, wherein the support comprises a polymethacrylate matrix onto which sulfoisobutyl groups have been grafted by covalent bonding, and b) eluting the virus from the support. | ||
| 地址 | Lyons FR | ||