GENE EXPRESSSION FROM MITOTICALLY STABLE EPISOMES

摘要

The invention provides a method of expressing a transgene in a host cell. The method comprises exposing a host cell, which comprises a genetic construct comprising a transgene, to conditions which substantially block mitosis in the host cell, such that the construct forms a mitotically stable episome resulting in expression of the transgene. The invention extends to the use of such constructs for expressing transgenes in a range of different medical and heterologous protein production applications.