Diagnosis of malignant neoplasms using anti-Notch3 antibodies

摘要

The present invention relates to novel antibodies that bind specifically to human Notch 3 and their use in the detection and/or diagnosis of Notch 3 related diseases, such as cancer. The present invention also includes nucleic acids encoding these novel antibodies, vectors and cell lines harboring the nucleic acids, and kits comprising the antibodies for use in the detection and diagnosis.

主权项

1. A method for diagnosing a malignant neoplasm expressing Notch3 protein in a subject, comprising contacting a sample from said subject with an antibody or fragment thereof which binds to Notch3 under conditions sufficient to bind Notch3, wherein an increase in Notch3 as compared to a normal noncancerous control is indicative of a malignant neoplasm wherein the antibody or fragment thereof comprises
(a) a variable light (VL) chain sequence comprising the amino sequence of SEQ ID NOs: 11, 12, and 13 or the amino sequence of SEQ ID NOs: 17, 18, and 19; and (b) a variable heavy (VH) chain sequence comprising the amino sequence of SEQ ID NOs: 18, 9, and 10 or the amino sequence of SEQ ID NOs: 14, 15, and 16.