发明名称 |
原核生物におけるグリコシル化されたタンパク質の発現方法 |
摘要 |
The present invention relates to a prokaryotic host cell comprising eukaryotic glycosyltransferase activity, where the eukaryotic glycosyltransferase activity is eukaryotic dolichyl-linked UDP-GlcNAc transferase activity and eukaryotic mannosyltransferase activity. Also disclosed is a method of producing a glycosylated protein by providing a prokaryotic host cell comprising the eukaryotic glycosyltransferase activity and culturing the prokaryotic host cell under conditions effective to produce a glycosylated protein. Another aspect of the present invention pertains to a method for screening bacteria or bacteriophages by expressing one or more glycans on the surface of a bacteria, attaching a label on the one or more glycans on the surface of the bacteria or on the surface of a bacteriophage derived from the bacteria, and analyzing the label in a high-throughput format. A glycosylated antibody comprising an Fv portion which recognizes and binds to a native antigen and an Fc portion which is glycosylated at a conserved asparagine residue is also disclosed. |
申请公布号 |
JP5667880(B2) |
申请公布日期 |
2015.02.12 |
申请号 |
JP20100541578 |
申请日期 |
2009.01.05 |
申请人 |
コーネル リサーチ ファンデーション インコーポレイテッド |
发明人 |
デリーサ マシュー;グァリーノ カサンドラ;マンセル トマス;フィッシャー アダム |
分类号 |
C12N1/21;C07K14/00;C07K16/00;C07K19/00;C12N15/09;C12P19/18;C12P21/00;C12Q1/04 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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