METHOD FOR ISOLATING POLYPHENOLS FROM OLIVE MILL WATER

摘要

The present invention relate to a highly efficient and novel method, using clean technologies, for obtaining a natural bioactive concentrate that is rich on polyphenols from olive mill water (OMW). The clean technologies integrate centrifugation, a drowning-out crystallization-based separation process, and vacuum evaporation. The method provides a highly-concentrated polyphenol isolate (up to 99% (mass fraction)) from other components presents in OMW, with up to half of the polyphenol content being hydroxytyrosol. The isolated polyphenols exhibit anti-oxidant, anti-microbial, anti-inflammatory, and anti-carcinogenic activities; they can be prepared as solid particles, as an aqueous solution, in an emulsion, or as lipidic-based nanoparticles. The isolated polyphenols can be used in the food industry, cosmetic industry, or pharmaceutical industry.

主权项

1. A method for isolating polyphenols from olive mill water (OMW) comprising the following sequential steps:
(a) obtaining an OMW after olive oil extraction from olive fruits; (b) pretreating the OMW by removing residual oil, large-sized organic compounds, particles, or soluble molecules from OMW thereby forming a more clear aqueous phase; (c) concentrating the aqueous phase thereby preparing a concentrate; (d) mixing the concentrate with solvent thereby producing a mixture; (e) transferring the mixture to a crystallization unit for isolating a liquid phase; and (f) transferring the liquid phase to a distillation unit for evaporating the solvent and for retaining a polyphenol-enriched aqueous solution.