| 摘要 |
FIELD: biotechnologies.SUBSTANCE: method involves cultivation of pertussis bacteria in liquid growth medium, separation of supernatant from microbial mass by centrifugation, separation of pertussis toxin from it, its detoxification with formalin in presence of saccharose in a thermostat, and sorption of anatoxin on an aluminium hydroxide gel. Strain Bordetella pertussis No. 287 is used as a producer of pertussis toxin. Detoxification of the above toxin Bordetella pertussis is performed during 19-20 days. The obtained anatoxin is combined with anatoxins of vaccine strains No. 203 and No. 305; they are taken in the ratio of 1:0.5:0.5 respectively; a physiological solution with a phosphate buffer is added to the mixture, and the mixture of anatoxins is absorbed on the aluminium hydroxide gel.EFFECT: invention allows improving activity and reducing toxicity of the obtained cell-free pertussis vaccine.3 tbl, 1 ex |
