Direct preparation of functional insulin producing cell from human dermal fibroblasts

摘要

PURPOSE: A method for directly producing functional insulin-producing cells from skin cells is provided to directly convert human somatic cells into insulin-producing cells without dedifferentiation into pluripotent cells and to obtain the insulin-producing cells which normally secrete insulin by glucose stimulation. CONSTITUTION: A method for producing insulin-producing cells comprises the step of promoting transcription of PDX-1 gene in somatic cells which do not produce insulin. The method directly converts the somatic cells into insulin-producing cells without dedifferentiation into cells with stemness. The step for promoting transcription of PDX-1 gene comprises the steps of transfecting cells by SMAD2 and transducing anti-Let7i by culturing the transfected cells for 2-10 days. [Reference numerals] (AA) Smad2 trait infection; (BB) Second day; (CC) FGM medium + 1% BSA; (DD) Anti-Let7i trait infection; (EE) EGM medium + 1% BSA; (FF) Seventh day; (GG) Glucose simulation index