发明名称 |
Recombinant microalgae cells producing novel oils |
摘要 |
Disclosed herein are obligate heterotrophic microalgae cells containing an exogenous gene. In some embodiments the gene is a sucrose utilization gene, and further disclosed are methods of manufacturing triglyceride oils using sugar cane or sugar beets as a feedstock in a heterotrophic fermentation. In other embodiments the feedstock is depolymerized cellulosic material. Also disclosed are cells that produce medium chain fatty acids at levels not produced in non-recombinant cells of the same species and genus. |
申请公布号 |
US8951777(B2) |
申请公布日期 |
2015.02.10 |
申请号 |
US201313852116 |
申请日期 |
2013.03.28 |
申请人 |
Solazyme, Inc. |
发明人 |
Franklin Scott;Somanchi Aravind;Espina Karen;Rudenko George;Chua Penelope |
分类号 |
C12N1/12;C12N15/82;C12N9/24;C12P7/64;C10L1/02;C12N9/00;C12N9/16;C12P7/20;C12N15/79;C07C1/207 |
主分类号 |
C12N1/12 |
代理机构 |
Alston & Bird LLP |
代理人 |
Alston & Bird LLP |
主权项 |
1. A method for preparing recombinant microalgal cells of the genus Prototheca, the method comprising:
heterotrophically cultivating recombinant microalgal cells of the genus Prototheca in a fermentation broth, the cells comprising a chromosomal knockout of a stearoyl ACP (acyl carrier protein) desaturase allele so that the cells produce an oil having an altered fatty acid profile comprising a reduction in unsaturated fatty acids; and isolating the cells from the broth. |
地址 |
South San Francisco CA US |