主权项 |
1. A method for making an aqueous hexane, aqueous diethyl ether, aqueous chloroform or aqueous ethanol extract of Gomphostema niveum leaf comprising air drying leaves of Gomphostema niveum to produce air dried leaves of Gomphostema niveum, powdering the air dried leaves of Gomphostema niveum to produce powder of leaves of Gomphostema niveum and subjecting the powder of leaves of Gomphostema niveum to extraction with water at reflux temperature, filtering the extract of leaves of Gomphostema niveum to produce a water extract of Gomphostema niveum, further extracting the water extract of Gomphostema niveum by liquid-liquid extraction using solvents selected from the group consisting of hexane, diethyl ether, chloroform and ethanol on the basis of increasing polarity to produce an aqueous hexane, aqueous diethyl ether, aqueous chloroform or aqueous ethanol extract, then the aqueous hexane. aqueous diethyl ether, aqueous chloroform or aqueous ethanol extract is concentrated to dryness under reduced pressure of about 150 psi and at a temperature of about 30° C. to produce the aqueous hexanes aqueous diethyl ether, aqueous chloroform or aqueous ethanol extract of Gomphostema niveum leaf. |