发明名称 |
MODIFIED BACILLUS CEREUS PHOSPHOLIPASE C PROTEIN AND METHOD OF PROCESSING VEGETABLE OIL |
摘要 |
A modified Bacillus cereus phospholipase C enzyme is provided, as well as a method of using the modified phospholipase C enzyme in a method of treating vegetable oil. In certain embodiments, this method may comprise combining a vegetable oil with an modified phospholipase C enzyme comprising an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO:1, wherein the amino acid residue at position 66 is a Trp (W) or Tyr (Y), and maintaining the combination under conditions suitable for the modified phospholipase C enzyme to catalyze the hydrolysis of phospholipids in the oil to produce diacylglycerol and a water soluble phosphate. |
申请公布号 |
WO2015017045(A1) |
申请公布日期 |
2015.02.05 |
申请号 |
WO2014US43294 |
申请日期 |
2014.06.19 |
申请人 |
KECLON SA |
发明人 |
CASTELLI, MARIA EUGENIA;MENZELLA, HUGO;PEIRU, SALVADOR;VETCHER, LEANDRO |
分类号 |
C12N9/20;C12N1/00;C12P7/44;C12P7/64 |
主分类号 |
C12N9/20 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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