MODIFIED &Agr;-HEMOLYSIN AND NANOPORE SEQUENCER USING SAME

摘要

Provided is a modified α-hemolysin in which a mutation is introduced to the amino acid sequence of an α-hemolysin monomer and which has arginine at the 147th position or glutamine at the 146th position from the N terminal of the amino acid sequence, and due to this modified α-hemolysin, the ability to form a nanopore in a lipid membrane is enhanced without reducing enzyme activity when compared to the wild form.