Methods for synthesizing and purifying aminoalkyl tetracycline compounds

摘要

Methods for the synthesis and purification of 9-amino alkyl tetracycline compounds are described.

主权项

1. A method of purifying an alkylaminomethyl minocycline compound, comprising:
a) injecting a low pH aqueous solution of said alkylaminomethyl minocycline compound into a liquid chromatography device in a polar organic solvent gradient, and combining the product fractions; b) adjusting the pH of said product fractions to 4.0-4.5; c) washing said product fractions with a first non-polar organic solvent to form a first organic layer and a first aqueous layer, and discarding said first organic layer; d) adjusting the pH of said first aqueous layer to 7.5-8.5; and e) washing said first aqueous layer with a second non-polar organic solvent to form a second organic layer and a second aqueous layer, and discarding said second aqueous layer, such that said alkylaminomethyl minocycline compound is purified.