METHOD FOR MEASURING CHANGES IN OPTICAL PROPERTIES OF WOUND TISSUE AND CORRELATING NEAR INFRARED ABSORPTION (FNIR) AND DIFFUSE REFLECTANCE SPECTROSCOPY SCATTERING (DRS) WITH TISSUE NEOVASCULARIZATION AND COLLAGEN CONCENTRATION TO DETERMINE WHETHER WOUND IS HEALING

摘要

PROBLEM TO BE SOLVED: To provide a method that enables a depth inspection of a tissue at greater depths, while simulating a clinical wound assess score.SOLUTION: Optical changes of tissue during wound healing measured by near infrared and diffuse reflectance spectroscopy are correlated with histologic changes, near infrared absorption coefficient are correlated with blood vessel in-growth over time, while diffuse reflectance spectroscopy (DRS) data is correlated with collagen concentration, thus, changes of optical properties of wound tissue at greater depths are also quantified by diffuse photon density wave (DPDW) methodology at near infrared wavelengths. A diffusion equation for semi-infinite media as a methodology is used to calculate the absorption and scattering coefficients based on measurements of phase and amplitude with a frequency domain or time domain device. In comparison to the non wounded sites, based on an increase in the absorption and scattering coefficients of the wounds and a decrease in blood saturation, a healing stage of the wound is determined.