摘要 |
PROBLEM TO BE SOLVED: To provide a method of easily observing a microorganism sample without complex pretreatment.SOLUTION: An environmental control scanning electron microscope having a schottky-type electron gun is used, to observe a microorganism sample, under the following conditions: under a saturated vapor pressure, in a temperature range of 0°C-5°C, with an acceleration voltage of 2 kV or more and 5 kV or less, and with an operation distance of 5 mm or more and 8 mm or less. |