| 摘要 |
The invention pertains to the use of C2-C6alpha-hydroxy acids selected from L-3-phenyl lactic acid, mucic (galactaric) acid, gluconic acid, glucaric acid, glyceric acid, 2-hydroxy butyric acid, alpha-hydroxyisovaleric acid, alpha-hydroxyisocaproic acid and erythronic acid and combinations thereof, as a growth- and production promoting ingredient, in culture media for culturing eukaryotic cells. The invention further pertains to culture media containing these alpha-hydroxy acid derivatives at levels of at least 0.001 mg/l. |
| 主权项 |
1. A process of producing a culture medium for culturing eukaryotic cells comprising adding to conventional culture medium ingredients, either as a pure substance or as a concentrate, one or more C2-C6 alpha-hydroxy acids selected from L-3-phenyl lactic acid, mucic (galactaric) acid, gluconic acid, glucaric acid, glyceric acid, 2-hydroxy butyric acid, alpha-hydroxyisovaleric acid, alpha-hydroxyisocaproic acid and erythronic acid, salts of these acids, esters of these acids and combinations thereof, as a growth-promoting and/or production-improving ingredient,such that the final concentration in the medium is at least 0.001 mg/l per individual alpha-hydroxy acid, its ester or its salt. |
