DIELECTRIC SPECTROSCOPY METHODS AND APPARATUS

摘要

Methods and apparatus are disclosed for correcting measurements received by applying a frequency-varying signal with a measuring device (e.g., a permittivity probe) to a population of living cells (e.g., contained in a bioreactor) and correcting measurement divergences using data acquired using an alternate analytical method (e.g., trypan blue exclusion). In one example, a method comprises receiving electrical property data for a first population of cells, the data obtained by applying a first frequency-varying signal to the population with a measuring device, receiving biological property data obtained using an alternate analytical technique, generating at least one value representative of the frequency dependence of the electrical property data, and determining a relationship between the biological property data and the representative value. In some examples, measurements of apoptosis are predicted using the electrical property data.

主权项

1. A method for determining a biological property of cells suspended in a liquid media, the method comprising:
receiving electrical property data obtained by applying a signal to said cells at two or more signal frequencies; receiving biological property data obtained using an alternate analytical technique; generating at least one value representative of frequency dependence of said electrical property data; and determining a relationship between said biological property data and said value representative of said electrical property data, wherein said value is generated by at least one or more of the following methods: by performing a multivariate analysis of said electrical property data, by determining the slope of a line corresponding to the electrical property data, by determining a ratio of the electrical property data measured at different frequencies, or by determining an area ratio under the curve derived from the electrical property data.