| 主权项 |
1. A family 5 glycoside hydrolase variant, the variant encoded by a mutated version of a wild-type parental polynucleotide having at least 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more or complete sequence identity to a (cDNA) nucleotide sequence of SEQ ID NO: 1 and comprising at least one of the following nucleotide residue changes:
the nucleotides at positions 157 to 159 are TCT; the nucleotides at positions 175 to 177 are AAG; the nucleotides at positions 175 to 177 are CAT; the nucleotides at positions 181 to 183 are CCT; the nucleotides at positions 190 to 192 are AAT; the nucleotides at positions 208 to 210 are ATG; the nucleotides at positions 259 to 261 are CAT; the nucleotides at positions 259 to 261 are ATG; the nucleotides at positions 274 to 276 are GCT; the nucleotides at positions 547 to 549 are TTG; the nucleotides at positions 550 to 552 are GAG; the nucleotides at positions 574 to 576 are AAT; the nucleotides at positions 589 to 591 are GCT; the nucleotides at positions 595 to 597 are ATG; the nucleotides at positions 598 to 600 are GTT; and the nucleotides at positions 898 to 900 are GCG;wherein the variant has at least one of the following activities: endoglucanase activity, a beta-mannanase activity, an exo-1,3-glucanase activity, an endo-1,6-glucanase activity, a xylanase activity, and an endoglycoceramidase activity; and wherein the activity of the variant is greater than a polypeptide encoded by the wild-type parental polynucleotide having a nucleotide sequence of SEQ ID NO: 1. |
