| 摘要 |
An assay for identifying ligands of the σ1 receptor based on a fusion protein comprising the σ1 receptor flanked by two fluorophores, so that said fluorophores are capable of producing constitutive FRET when no ligand is bound to the σ1 receptor. The fusion protein of the invention also allows the simultaneous determination whether the newly determined ligand is an agonist or an antagonist. Said fusion protein has been expressed in a cell, giving rise to a new cellular model useful for identifying σ1 receptor ligands, and additionally for discriminating between agonists and antagonists. |
