METHOD FOR PRODUCING CELL HAVING NUCLEIC ACID INTRODUCED THEREIN

摘要

A method for producing a cell having nucleic acid introduced therein and having low cytotoxicity is useful in primary cells and slowly dividing cells, for which nucleic acid introduction is difficult with conventional techniques. A method for introducing nucleic acid into a cell includes the steps of mixing a nucleic acid with a temperature sensitive material at a temperature lower than the cloud point of the temperature sensitive material, the temperature sensitive material being formed by adding 2-amino-2-hydroxymethyl-1,3-propanediol to a temperature-sensitive polymer material having 2-N,N-dimethylaminoethyl methacrylate and/or a derivative thereof as the main polymer component, flow coating a culture vessel with the resulting mixed liquid, and culturing a cell suspension provided to the culture vessel at a temperature higher than the cloud point.

主权项

1. A method for producing a cell having nucleic acid introduced therein, comprising the steps of:
producing a gene transfer composition by mixing a nucleic acid with an aqueous solution of a temperature sensitive composition at a temperature lower than a cloud point of the temperature sensitive composition, the temperature sensitive composition being formed by adding 2-amino-2-hydroxymethyl-1,3-propanediol to a temperature-sensitive polymer material having 2-N,N-dimethylaminoethyl methacrylate and/or a derivative thereof as a main polymer component; adhering the gene transfer composition to a culture vessel; and culturing a cell suspension provided to the culture vessel at a temperature higher than the cloud point.