| 发明名称 |
METHODS AND SYSTEMS FOR TRACKING BIOREMEDIATION PROCESSES |
| 摘要 |
A method for expression of engineered constructs containing sequences coding for functional genes of interest in E. coli strains. Here tceA and tceB are fused to an inducible, active promoter to optimize transcription of the open reading frames, and an introduced consensus Shine-Dalgarno sequence allowing for optimal ribosome binding and translation of the open reading frames, and codons optimized for expression in E. coil. This over-expressed protein is then used to design appropriate mass spectro metric methods for environmental detection. |
| 申请公布号 |
US2015010945(A1) |
申请公布日期 |
2015.01.08 |
| 申请号 |
US201214127298 |
申请日期 |
2012.06.19 |
| 申请人 |
Krajmalnik-Brown Rosa;Halden Rolf U.;Wilson James W. |
发明人 |
Krajmalnik-Brown Rosa;Halden Rolf U.;Wilson James W. |
| 分类号 |
C12N15/70;H01J49/00;H01J49/04;C07K14/195 |
主分类号 |
C12N15/70 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method for expression of engineered constructs containing sequences coding for functional genes of interest in E. coli strains comprising:
fusing genes of interest to an inducible, active promoter to optimize transcription of the open reading frames, and an introduced consensus Shine-Dalgarno sequence allowing for optimal ribosome binding and translation of the open reading frames, and codons optimized for expression in E. coil. |
| 地址 |
Chandler AZ US |
