发明名称 |
Method of controlling cleavage by OmpT protease |
摘要 |
Methods of using OmpT protease are provided. In particular, the invention provides for a method of controlling cleavage of a polypeptide by OmpT protease comprising, converting a sequence site comprising two arbitrary consecutive amino acids and/or amino acid(s) in the vicinity of the site in the polypeptide into other amino acids; which method comprises setting lysine or arginine as the amino acid at the -1-position concerning the site and setting a specific amino acid as the amino acid at the +1-position; and/or setting specific amino acid(s) as the amino acid(s) at the -4-position and/or the -6-position relative to the site; so that a desired part of the polypeptide is cleaved by OmpT protease and/or an undesired part of the polypeptide is not (or hardly) cleaved by OmpT protease. A method of producing a target polypeptide is also provided.
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申请公布号 |
US7344856(B1) |
申请公布日期 |
2008.03.18 |
申请号 |
US20000674777 |
申请日期 |
2000.03.03 |
申请人 |
ASUBIO PHARMA CO. LTD. |
发明人 |
OKUNO KAZUAKI;YABUTA MASAYUKI;OHSUYE KAZUHIRO |
分类号 |
C12P21/06;C07K14/58;C07K14/585;C07K14/605;C12N1/20;C12N9/48;C12N15/00;C12N15/62;C12N15/63;C12P21/00 |
主分类号 |
C12P21/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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