TEMPERATURE SENSITIVE MUTANTS OF MATRIX METALLOPROTEASE 1 AND USE THEREOF

摘要

PROBLEM TO BE SOLVED: To provide modified matrix metalloprotease (MMP) enzymes exhibiting temperature-dependent activity and use thereof.SOLUTION: A modified MMP1 having at least one amino acid replacement which provides increased activity in comparison with an MMP-1 polypeptide having no amino acid replacement, where the modified MMP1 polypeptide is a mature enzyme that is a zymogen processed to lack prosegment or proregion of the enzyme. For example, they can be used to treat ECM-mediated diseases or disorders characterized by increased deposition or accumulation of one or more ECM components.