METHOD FOR THE DETERMINATION OF RENAL FUNCTION

摘要

Immunoassay for the detection of CAF (22-Kd fragment of agrin) in a sample, which additionally can include larger agrin-fragments (110-Kd fragment) and agrin itself and in which the CAF to be detected can be present in one or both of the following 2 different conformations CAF-closed and CAF-open, wherein in an optional first step the larger fragments and agrin possibly present in the sample are removed therefrom, in a second step the remaining portion of the sample obtained after possible removal of the larger fragments in the first step is incubated with a (primary) antibody with known binding properties to the different conformations of the CAF, with the antibody being capable to specifically bind to at least the CAF-open conformation, and in a third step it is determined whether or not antibody-CAF-complexes have been formed after incubation.