发明名称 |
METHOD OF PRODUCING BIOLOGICALLY ACTIVE VITAMIN K DEPENDENT PROTEINS BY RECOMBINANT METHODS |
摘要 |
The invention relates to commercially viable methods for producing biologically active vitamin K dependent proteins, particularly Factor IX. Factor IX is produced at a level of at least about 15mg/L and is at least 25% biologically active. The method relies upon co-expression of one or more of paired basic amino acid converting encyme (PACE), vitamin K dependent epoxide reductase (VKOR) and vitamin K dependent ³-glutamyl carboxylase (VKGC) at a preferred ratio so that the vitamin K dependent protein is efficiently produced and processed by a recombinant cell. |
申请公布号 |
SI1969127(T1) |
申请公布日期 |
2014.12.31 |
申请号 |
SI20060031827T |
申请日期 |
2006.12.21 |
申请人 |
CNJ HOLDINGS, INC;UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL |
发明人 |
DROHAN WILLIAM N.;GRIFFITH MICHAEL J.;TAYLOR JOHN R.;STAFFORD DARREL W. |
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