Method for identifying olfactory receptor included in one olfactory cell

摘要

The present invention provides a novel method for identifying an olfactory receptor included in one olfactory cell. In the present invention, amplified is the cDNA derived from the mRNA of the one olfactory cell by a PCR method using a forward primer represented by SEQ ID: 01 and a reverse primer represented by SEQ ID: 02. Subsequently, determined is whether or not a gene sequence of the amplified cDNA is identical to one gene sequence included in gene sequences coding for olfactory receptors included in the mouse olfactory receptor group A. Finally, determined is that the olfactory receptor included in the one olfactory cell is the olfactory receptor corresponding to the one gene sequence which is identical to the gene sequence of the cDNA in the previous step, if the gene sequence of the cDNA is identical to the one gene sequence in the previous step.

主权项

1. A method for identifying an olfactory receptor included in one olfactory cell among a mouse olfactory receptor group A, the method comprising steps of:
(a) obtaining the one olfactory cell; (b) extracting mRNA from the one olfactory cell obtained in the step (a); (c) obtaining cDNA with reverse transcriptase from the mRNA extracted in the step (b); (d) amplifying the cDNA obtained in the step (c) by a PCR method using a forward primer represented by SEQ ID NO: 01 and a reverse primer represented by SEQ ID NO: 02, wherein
SEQ ID NO: 01 is A (adenine)-T (thymine)-G (guanine)-G (guanine)-C (cytosine)-I (inosine)-T (thymine)-A (adenine)-Y (thymine or cytosine)-G (guanine)-A (adenine)-Y (thymine or cytosine)-M (adenine or cytosine)-G (guanine)-I (inosine)-T (thymine)-A (adenine)-Y (thymine or cytosine)-G (guanine)-T (thymine)-I (inosine)-G (guanine)-C (cytosine); andSEQ ID NO: 02 is T (thymine)-C (cytosine)-I (inosine)-C (cytosine)-G (guanine)-R (guanine or adenine)-T (thymine)-T (thymine)-Y (thymine or cytosine)-C (cytosine)-T (thymine)-I (inosine)-A (adenine)-G (guanine)-R (guanine or adenine)-C (cytosine)-T (thymine)-R (guanine or adenine)-T (thymine)-A (adenine)-I (inosine)-A (adenine)-T (thymine)-R (guanine or adenine)-A (adenine)-A (adenine)-I (inosine)-G (guanine)-G (guanine)-R (guanine or adenine)-T (thymine)-T (thymine); (e) determining the sequence of the cDNA amplified in the step (d); and (f) determining whether or not the gene sequence of the cDNA amplified in step (d) is identical to one gene sequence included in gene sequences coding for olfactory receptors included in the mouse olfactory receptor group A, wherein the mouse olfactory receptor group A is selected from the group consisting of: Olfr1, Olfr20, Olfr27, Olfr39, Olfr43, Olfr44, Olfr51, Olfr57, Olfr75-ps1, Olfr156, Olfr186, Olfr195, Olfr204, Olfr255, Olfr341, Olfr346, Olfr356, Olfr357, Olfr376, Olfr377-ps1, Olfr385, Olfr389, Olfr390, Olfr391-ps1, Olfr395, Olfr397, Olfr408-ps1, Olfr459, Olfr478, Olfr482, Olfr508, Olfr701, Olfr711, Olfr768, Olfr779, Olfr790, Olfr829, Olfr844, Olfr847, Olfr850, Olfr854, Olfr868, Olfr943, Olfr944, Olfr951, Olfr954, Olfr965, Olfr971, Olfr992, Olfr1016, Olfr1046, Olfr1049, Olfr1099, Olfr1101, Olfr1171-ps1, Olfr1357, Olfr1402, Olfr1425, Olfr1491, Olfr1496, Olfr1501, and Olfr1509.