Methods of Preparing Cells and Compositions

摘要

The present invention provides a method of preparing adult red blood cells from stem cells in vitro using certain transcription factors for use in medicine, transfusions and transplants. The invention also provides blood compositions with cells prepared by the method.

主权项

1. A method of preparing adult red blood cells in culture comprising the steps of obtaining stem cells or cell lines from a source, culturing said cells in a defined medium, modifying the cells with the transcription factors BCL11A and EKLF and converting fetal globin in the cells into adult globin.