Diagnosis and treatment of Alzheimer's disease

摘要

Methods are provided for the prevention, treatment and diagnosis of Alzheimer's disease, based on the glycosylation pattern of amyloid-beta peptides in body fluids and tissues.

主权项

1. An in vitro method for diagnosing or prognosing Alzheimer's disease in a subject, or determining whether a subject is at increased risk of developing Alzheimer's disease, comprising the steps of:
a) obtaining a sample from the subject; b) enriching for Abeta peptides in the sample; c) separating the Abeta peptides of step b) using liquid chromatography; d) identifying Abeta peptide fragments by mass spectrometry; e) measuring the level of O-linked glycosylation at Tyr10 of the Abeta peptides; and f) utilizing information on the level of O-linked glycosylation at Tyr10 of the Abeta peptides in the sample as compared to a reference value representing a known disease or health status to diagnose or prognose Alzheimer's disease in the subject, or to determine whether there is an increased risk of Alzheimer's disease in the subject.