ELECTROSPUN FISH COLLAGEN/SYNTHETIC POLYMER HYBRID NANOFIBER SCAFFOLDS, METHOD THEREOF AND USE THEREOF

摘要

The present invention provides a method for producing a nanofiber support and a fish collagen/synthetic polymer composite nanofiber support produced through the method, wherein the method comprises a first step of dissolving a synthetic polymer in an organic solvent; a second step of producing a fish collagen solution by dissolving fish collagen in water; a third step of adding and mixing the synthetic polymer solution produced from the first step in the fish collagen solution produced from the second step; and a fourth step of producing a nanofiber support by electrospinning the mixture produced from the third step. The composite nanofiber support can exhibit higher mechanical stability than existing nanofiber supports including synthetic polymer ingredients and constitutes a cell culturing environment similar to that of a living body, wherein three-dimensional cell culturing efficiency such as cell adhesion, cellular viability, and cell distributing efficiency is remarkably improved, and an excellent biomimetic function is exhibited so that the same is appropriate as a three-dimensional cell-culturing support. Accordingly, a three-dimensional cell-culturing method using the fish collagen/synthetic polymer nanofiber support of the present invention is appropriate as a three-dimensional cell-culturing model which can be used in testing drug sensitivity and studying the formation, growth, spread, and death of cancer cells. The same can be used in studying the development of a biological tissue which can recover or replace a part or all of bones, cartilages, blood vessels, bladder, skin, and tissues such as muscles.