| 主权项 |
1. A method of detecting a genome DNA methylation, comprising following steps:
1) digesting a genome DNA sample with MspJI, to obtain fragments, 2) sequencing the fragments, to obtain reads; 3) aligning the reads to a reference sequence, to select a uniquely aligned read; and 4) determining a site in the reference sequence being methylated in the uniquely aligned read; wherein the site in the reference sequence corresponds to a C site in at least one of YNCGNR, YCNGR, CNNG, GNNC, CYNRG, CNYRNG, YNNGCNNR, YNNGNCNNR, CNNR, YNNG and a complementary strand thereof, wherein Y is C or T, R is A or G, N is A, C, T or G, and H is C, A or T. |
