WIDE-FIELD MICROSCOPY USING SELF-ASSEMBLED LIQUID LENSES

摘要

A method of imaging a sample includes depositing a droplet containing the sample on a substrate, the sample having a plurality of particles contained within a fluid. The substrate is then tilted to gravitationally drive the droplet to an edge of the substrate while forming a dispersed monolayer of particles having liquid lenses surrounding the particles. A plurality of lower resolution images of the particles contained on the substrate are obtained, wherein the substrate is interposed between an illumination source and an image sensor, wherein each lower resolution image is obtained at discrete spatial locations. The plurality of lower resolution images of the particles are converted into a higher resolution image. At least one of an amplitude image and a phase image of the particles contained within the sample is then reconstructed. In some embodiments, only a single lower resolution image may be sufficient.