METHOD FOR INDUCING DIFFERENTIATION OF HUMAN PLURIPOTENT STEM CELLS INTO INTERMEDIATE MESODERM CELLS

摘要

The present invention provides a method for inducing differentiation of a human pluripotent stem cell into an intermediate mesoderm cell without the use of a growth factor. Such method comprises performing culture in a medium containing a specific compound, so as to induce differentiation of a human pluripotent stem cell into an intermediate mesoderm cell.

主权项

1. A method for producing an intermediate mesoderm cell from a human pluripotent stem cell, the method comprising:
(i) culturing a human pluripotent stem cell in a medium comprising a GSK-3 beta inhibitor or a GSK-3 beta inhibitor and a retinoic acid derivative; and (ii) culturing the cell obtained in the culturing (i) in a medium comprising a retinoic acid derivative.