Method for identifying, expanding and removing adult stem cells and cancer stem cells

摘要

Described are the use of stem cell markers for the isolation of stem cells and the use of the obtained stem cells in, for example, research or treatment, such as for the preparation of a medicament for the treatment of damaged or diseased tissue. In one aspect, provided is a method for obtaining or isolating stem cells that comprises preparing a cell suspension from a tissue or organ sample, contacting the cell suspension with an Lgr 6 or 5 binding compound, identifying the cells bound to the binding compound, and isolating the stem cells from the binding compound. Further described are methods and compositions for cancer treatment, for example, by eradicating cancer stem cells.

主权项

1. A method for obtaining stem cells from a cell suspension, the method comprising:
providing a cell suspension; contacting said cell suspension with an antibody specific for Lgr5 so as to bind the antibody specific for Lgr5 to Lgr5 positive cells in the cell suspension; and at least partially purifying cells bound to the antibody specific for Lgr5 from cells not bound to the antibody specific for Lgr5 from the cell suspension so as to obtain stem cells.