DESIGNING PADLOCK PROBES FOR TARGETED GENOMIC SEQUENCING

摘要

Methods, systems, and computer programs for designing probes or primers for nucleic acid sequencing, generating libraries of nucleic acid sequences, and mapping genomic sequences are provided herein,

主权项

1. A method of designing probes or primers for sequencing a target nucleic acid molecule, comprising:
selecting one or more inputs associated with efficiency of the probe or primer; selecting a target nucleic acid sequence; generating a first library of probe or primer sequences that comprise a target capturing sequence that is complementary to the target nucleic acid sequence; determining the efficiency of each probe or primer sequence in the first library by using an algorithm comprising the one or more selected inputs; ranking the probe or primer sequences in the first library by efficiency; extracting the probe or primer sequences having the highest efficiency to generate a second library; and adding a linker sequence to each of the probe or primer sequences in the second library.