Multicomponent or monocomponent vaccine to be used against Chagas disease, pharmaceutical compositions containing them, procedure for the obtention of immunogen of said vaccines, and nucleic acid used in said procedure

摘要

A vaccine against the Chagas disease, capable of stimulating the immune response against the trans-sialidase virulence factor of the Trypanosoma cruzi parasite, which is a multicomponent vaccine comprising: (a) an immunogenic portion formed by one or more recombinant or synthetic polypeptides or fractions of thereof and (b) one or more polynucleotides including the regions codifying one or more immunogenic polypeptides, or a monocomponent vaccine comprising at least one component selected among an immunogenic portion formed by one or more recombinant or synthetic polypeptides or fractions of them and a group of polynucleotides including the regions codifying one or more immunogenic polypeptides derived from Trypanosoma cruzi and pharmaceutical compositions containing said multicomponent and monocomponent vaccines, the procedures for obtaining the immunogen portion of said vaccines and the nucleic acid used in the procedure.

主权项

1. A multicomponent vaccine effective to protect against, eliminate, or moderate the clinical consequences of Chages disease, said vaccine comprising, as active ingredient,
an immunogenic component made up of one or more recombinant or synthetic polypeptides, wherein the immunogenic component is selected from one or more polypeptides, the structure of which includes a C-terminal region composed of at least two repetitive units of amino acids; wherein each repetitive unit shows the following amino acid sequence: AHSTPSTPVDSSand wherein a polypeptide with trans-sialidase activity of Trypanosoma cruzi is fused to the C-terminal region, and
which comprises an adjuvant that does not inhibit trans-sialidase enzymatic activity of the immunogen portion, wherein the adjuvant is aluminum oxide.