Methods of bead manipulation and forming bead arrays

摘要

According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.

主权项

1. A method of preparing a sequencing apparatus, the method comprising:
applying a solution to a substrate including a plurality of microwells exposed at a surface of the substrate, a plurality of polynucleotide beads depositing at the surface of the substrate, a first portion of the plurality of polynucleotide beads depositing into microwells of the plurality of microwells, a second portion of the plurality of polynucleotide beads depositing between microwells of the plurality of microwells, the solution comprising a plurality of inert beads to dislodge the second portion of the polynucleotide beads from between the microwells; and removing the solution including the plurality of inert beads and the second portion of the plurality of polynucleotide beads.