Production of mevalonate, isoprene, and isoprenoids using genes encoding polypeptides having thiolase, HMG-CoA synthase and HMG-CoA reductase enzymatic activities

摘要

The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in microorganisms via the heterologous expression of the mvaE and mvaS genes from the organisms Listeria grayi DSM 20601, Enterococcus faecium, Enterococcus gallinarum EG2, and Enterococcus casseliflavus.

主权项

1. Recombinant cells with increased production of isoprene, the cells comprising one or more heterologous nucleic acids comprising nucleotide sequences selected from the group consisting of:
a. a mevalonate E (mvaE) gene and a mevalonate S (mvaS) gene from Enterococcus gallinarum (E. gallinarum); b. a mvaE gene and a mvaS gene from Enterococcus casseliflavus (E. casseliflavus); c. a mvaE gene and a mvaS gene from Enterococcus faecium (E. faecium); and d. a mvaE gene and a mvaS gene from Listeria grayi (L. grayi)wherein said mvaE gene and mvaS gene encode polypeptides having thiolase, HMG-CoA synthase, and HMG-CoA reductase catalytic activities, and wherein the cells further comprise:
i. one or more nucleic acids encoding polypeptides of the lower MVA pathway; and ii. a heterologous nucleic acid encoding an isoprene synthase polypeptide,wherein the cells produce increased amounts of isoprene compared to isoprene-producing cells that (A) contain an mvaE gene and mvaS gene from Enterococcus faecalis (E. faecalis) and (B) do not contain said mvaE gene and mvaS gene from E. gallinarum, E. casseliflavus, E. faecium, or L. grayi.