Method for the Characterization of Microorganisms on Solid or Semi-Solid Media

摘要

The present invention relates to methods and systems for scanning, detecting, and monitoring microorganisms on solid or semi-solid media using intrinsic fluorescence (IF) measurements. The methods are further directed to detection, characterization and/or identification of microorganisms on a solid or semi-solid media using intrinsic fluorescence (IF) measurements that are characteristic of said microorganisms.

主权项

1. A method of detecting and characterizing a microorganism on a solid or semi-solid medium, comprising:
(a) interrogating one or more colonies located on a solid or semi-solid medium wherein the excitation beam is smaller in diameter than the colony to be interrogated, to produce intrinsic fluorescence (IF) measurements characteristic of a microorganism in said colony; and (b) identifying and/or characterizing the microorganism in the colony based on said intrinsic fluorescence (IF) measurements.