MICROBEAD ANALYSIS METHOD AND MICROBEAD ANALYZER

摘要

Provided is a microbead analysis method for a microbead. The microbead is formed in a columnar shape having a top surface and a bottom surface facing each other, as placed almost in parallel, and a side surface extending therefrom, and carries an identification pattern formed on at least one of the top surface and the bottom surface and a substance immobilized on a surface thereof having affinity to an analyte substance. The method includes detecting fluorescence emitted from the microbead surface due to interaction of the analyte substance with the substance having affinity to the analyte substance from a region including a region of the top surface and the bottom surface where there is no identification pattern formed and the side surface.

主权项

1. A microbead analyzer for use in analysis of a microbead carrying an identification pattern on a surface thereof, comprising:
an image-acquiring means for obtaining a transmission image and a fluorescence image of the microbead; means for detecting the identification pattern from the transmission image; and means for detecting fluorescence from a region where the identification pattern of the microbead is not formed in the fluorescence image.