| 摘要 |
The present invention relates to an image processor, a program and a microscope, which enable a TIRF image and a confocal image to be superposed simply and accurately. A reference point detection unit 111 detects, reference points, three or more images respectively from a TIRF image of a predetermined surface of a sample obtained using a total internal reflection fluorescence microscope, and a confocal image of the predetermined surface of the sample obtained using a confocal microscope, the three or more images in the TIRF image respectively corresponding to the three or more images in the confocal image as images of a same position on the predetermined surface of the sample. The coordinate transformation coefficient calculation unit 112 calculates coordinate transformation coefficients for mutually transforming a coordinate system of the reference points in the TIRF image and a coordinate system of the reference points in the confocal image. A superposing unit 113 superposes the TIRF image and the confocal image using the coordinate transformation coefficient. The present invention can be applied to, for example, an image processor which processes an observation image of a microscope. |
